FilterSeq

Filters sequences in FASTA/FASTQ files

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

--version

show program’s version number and exit

output files:

<command>-pass

reads passing filtering operation and modified accordingly, where <command> is the name of the filtering operation that was run.

<command>-fail

raw reads failing filtering criteria, where <command> is the name of the filtering operation.

output annotation fields:

None

FilterSeq length

Filters reads by length.

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

-s <seq_files>

A list of FASTA/FASTQ files containing sequences to process.

--fasta

Specify to force output as FASTA rather than FASTQ.

--failed

If specified create files containing records that fail processing.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

-n <min_length>

Minimum sequence length to retain.

--inner

If specified exclude consecutive missing characters at either end of the sequence.

FilterSeq maskqual

Masks low quality positions.

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

-s <seq_files>

A list of FASTA/FASTQ files containing sequences to process.

--fasta

Specify to force output as FASTA rather than FASTQ.

--failed

If specified create files containing records that fail processing.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

-q <min_qual>

Quality score threshold.

FilterSeq missing

Filters reads by N or gap character count.

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

-s <seq_files>

A list of FASTA/FASTQ files containing sequences to process.

--fasta

Specify to force output as FASTA rather than FASTQ.

--failed

If specified create files containing records that fail processing.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

-n <max_missing>

Threshold for fraction of gap or N nucleotides.

--inner

If specified exclude consecutive missing characters at either end of the sequence.

FilterSeq quality

Filters reads by quality score.

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

-s <seq_files>

A list of FASTA/FASTQ files containing sequences to process.

--fasta

Specify to force output as FASTA rather than FASTQ.

--failed

If specified create files containing records that fail processing.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

-q <min_qual>

Quality score threshold.

--inner

If specified exclude consecutive missing characters at either end of the sequence.

FilterSeq repeats

Filters reads by consecutive nucleotide repeats.

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

-s <seq_files>

A list of FASTA/FASTQ files containing sequences to process.

--fasta

Specify to force output as FASTA rather than FASTQ.

--failed

If specified create files containing records that fail processing.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

-n <max_repeat>

Threshold for fraction of repeating nucleotides.

--missing

If specified count consecutive gap and N characters ‘ in addition to {A,C,G,T}.

--inner

If specified exclude consecutive missing characters at either end of the sequence.

FilterSeq trimqual

Trims sequences by quality score decay.

usage: FilterSeq [-h] [–version] ...

-h, --help

show this help message and exit

-s <seq_files>

A list of FASTA/FASTQ files containing sequences to process.

--fasta

Specify to force output as FASTA rather than FASTQ.

--failed

If specified create files containing records that fail processing.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

-q <min_qual>

Quality score threshold.

--win <window>

Nucleotide window size for moving average calculation.

--reverse

Specify to trim the head of the sequence rather than the tail.