Workflows

• Roche 454 BCR mRNA with Multiplexed Samples
  • Overview of Experimental Data
  • Overview of the Workflow
  • Quality control
  • Sample barcode and primer identification
  • Deduplication and filtering
  • Output files
  • Performance
• Illumina MiSeq 2x250 BCR mRNA
  • Overview of Experimental Data
  • Overview of the Workflow
  • Paired-end assembly
  • Quality control and primer annotation
  • Deduplication and filtering
  • Output files
  • Performance
• UMI Barcoded Illumina MiSeq 2x250 BCR mRNA
  • Overview of Experimental Data
  • Overview of the Workflow
  • Quality control, UMI annotation and primer masking
  • Generation of UMI consensus sequences
  • Paired-end assembly of UMI consensus sequences
  • Deduplication and filtering
  • Output files
  • Performance